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1. (WO2018103502) IL-6R KNOCKOUT CAR-T TRANSGENIC VECTOR FOR ALLEVIATING CRS, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME

Pub. No.:    WO/2018/103502    International Application No.:    PCT/CN2017/110654
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Tue Nov 14 00:59:59 CET 2017
IPC: C12N 15/113
C12N 15/867
C12N 15/66
A61K 35/17
A61K 48/00
A61P 29/00
Applicants: SHANGHAI UNICAR-THERAPY BIO-MEDICINE TECHNOLOGY CO., LTD
上海优卡迪生物医药科技有限公司
Inventors: QI, Wei
祁伟
YU, Lei
俞磊
KANG, Liqing
康立清
YU, Zhou
余宙
Title: IL-6R KNOCKOUT CAR-T TRANSGENIC VECTOR FOR ALLEVIATING CRS, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME
Abstract:
Provided is a CAR-T transgenic vector for alleviating, via IL-6R knockout, cytokine release syndrome (CRS). The vector comprises: an ampicillin resistance gene sequence; a prokaryotic replicon pUC Ori sequence; a viral replicon SV40 Ori sequence; an enhanced woodchuck hepatitis virus posttranscriptional regulatory element (eWPRE); a human EF1α promoter; a lentivirus packaging cis-element for lentivirus packaging; a humanized IL-6R single-chain antibody IL6RscFv1/2/3; an IRES ribosome binding sequence; an IL-6 signal peptide; a humanized antibody Fc fragment; and a second generation or third generation chimeric antigen receptor (CAR) combining the functions of identification, transfer, and activation. Further provided is a method for preparing the vector and an application of the vector in preparing a pharmaceutical drug for alleviating CRS.