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|1. (WO2018103502) IL-6R KNOCKOUT CAR-T TRANSGENIC VECTOR FOR ALLEVIATING CRS, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME|
|Applicants:||SHANGHAI UNICAR-THERAPY BIO-MEDICINE TECHNOLOGY CO., LTD
|Title:||IL-6R KNOCKOUT CAR-T TRANSGENIC VECTOR FOR ALLEVIATING CRS, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME|
Provided is a CAR-T transgenic vector for alleviating, via IL-6R knockout, cytokine release syndrome (CRS). The vector comprises: an ampicillin resistance gene sequence; a prokaryotic replicon pUC Ori sequence; a viral replicon SV40 Ori sequence; an enhanced woodchuck hepatitis virus posttranscriptional regulatory element (eWPRE); a human EF1α promoter; a lentivirus packaging cis-element for lentivirus packaging; a humanized IL-6R single-chain antibody IL6RscFv1/2/3; an IRES ribosome binding sequence; an IL-6 signal peptide; a humanized antibody Fc fragment; and a second generation or third generation chimeric antigen receptor (CAR) combining the functions of identification, transfer, and activation. Further provided is a method for preparing the vector and an application of the vector in preparing a pharmaceutical drug for alleviating CRS.