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1. (WO2018103148) METHOD FOR RAPID CONSTRUCTION OF PETROLEUM DEGRADING STRAIN USING MICROBIAL INTRACELLULAR ENZYME

Pub. No.:    WO/2018/103148    International Application No.:    PCT/CN2016/112025
Publication Date: Fri Jun 15 01:59:59 CEST 2018 International Filing Date: Tue Dec 27 00:59:59 CET 2016
IPC: C12Q 1/04
C12R 1/01
Applicants: INSTITUTE OF APPLIED ECOLOGY CHINESE ACADEMY OF SCIENCES (IAE CAS)
中国科学院沈阳应用生态研究所
Inventors: GUO, Shuhai
郭书海
WANG, Jianing
王佳宁
WANG, Sa
王卅
ZHANG, Qiang
张强
LI, Fengmei
李凤梅
Title: METHOD FOR RAPID CONSTRUCTION OF PETROLEUM DEGRADING STRAIN USING MICROBIAL INTRACELLULAR ENZYME
Abstract:
Provided is a method for rapid screening and construction of functional strains for repairing petroleum contaminated soil using a microbial intracellular enzyme. By detecting enzymatic reaction products, petroleum hydrocarbon macromolecular (C23-C35) metabolic strains and small molecule (C10~C22) metabolic strains are distinguished, and combined in stages. The application method of composite strains can be quickly optimized, thereby achieving construction of efficient degrading composite strains by detecting the effects of the intracellular enzymes.