A backplane (1) and a computer system comprising the backplane (1). The backplane (1) comprises a backplane main body. The backplane main body is provided with four first card interface regions (10, 20, 30) and eight second card interface regions (40). The four first card interface regions (10, 20, 30) and the eight second card interface regions (40) are uniformly arranged in four rows on the backplane main body, and the length of each first card interface region is two times of the length of each second card interface region, so as to form an interface region properly arranged. The backplane has good expandability and the extensibility.