Search International and National Patent Collections

1. (WO2018102818) PROCESSIVE TEMPLATE INDEPENDENT DNA POLYMERASE VARIANTS

Pub. No.:    WO/2018/102818    International Application No.:    PCT/US2017/064517
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Tue Dec 05 00:59:59 CET 2017
IPC: C07H 21/04
C12N 9/12
C12P 19/30
C12P 19/34
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors: GRISWOLD, Kettner John Frederick, Jr.
TURCZYK, Brian M.
WIEGAND, Daniel Jordan
CHURCH, George M.
GARRUSS, Alexander
Title: PROCESSIVE TEMPLATE INDEPENDENT DNA POLYMERASE VARIANTS
Abstract:
An enzymatic method of making a polynucleotide is provided. The method includes combining a selected nucleotide triphosphate, one or more cations, a template-independent polymerase, and an associated processivity factor in an aqueous reaction medium including a target substrate comprising an initiator sequence and having a 3' terminal nucleotide attached to a single stranded portion, such that the template-independent polymerase and the associated processivity factor interact with the target substrate under conditions which covalently add one or more of the selected nucleotide triphosphate to the 3' terminal nucleotide. Also provided are mutant template-independent polymerases having a processivity factor attached thereto.