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1. (WO2018102816) SIMULTANEOUS GENE EDITING AND HAPLOID INDUCTION

Pub. No.:    WO/2018/102816    International Application No.:    PCT/US2017/064512
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Tue Dec 05 00:59:59 CET 2017
IPC: A01H 1/00
C12N 9/22
C12N 15/09
C12N 15/63
C12Q 1/68
Applicants: SYNGENTA PARTICIPATIONS AG
SYNGENTA CROP PROTECTION LLC
Inventors: KELLIHER, Timothy
QUE, Qiudeng
Title: SIMULTANEOUS GENE EDITING AND HAPLOID INDUCTION
Abstract:
The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.