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1. (WO2018102797) SRM/MRM ASSAYS FOR CD56 AND CHGA PROTEINS

Pub. No.:    WO/2018/102797    International Application No.:    PCT/US2017/064406
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Tue Dec 05 00:59:59 CET 2017
IPC: C07K 14/575
C07K 14/705
G01N 33/68
Applicants: EXPRESSION PATHOLOGY, INC.
Inventors: HEMBROUGH, Todd
CECCHI, Fabiola
SCHWARTZ, Sarit
SCOTT, Kerry
Title: SRM/MRM ASSAYS FOR CD56 AND CHGA PROTEINS
Abstract:
Methods are provided for quantifying CD56 and CHGA proteins directly in formalin- fixed biological samples by Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological samples may include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, and FFPE tissue blocks and cells from those blocks. A protein sample may be prepared from said biological sample using the Liquid Tissue reagents and protocol and a designated protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one peptide fragment derived from each of the proteins.