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1. (WO2018102473) METHODS AND MATERIALS FOR CLONING FUNCTIONAL T CELL RECEPTORS FROM SINGLE T CELLS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/102473 International Application No.: PCT/US2017/063813
Publication Date: 07.06.2018 International Filing Date: 29.11.2017
IPC:
C07K 14/725 (2006.01) ,C12N 15/85 (2006.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
725
T-cell receptors
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants: SHLOMCHIK, Mark[US/US]; US
PANOUSIS, Constantinos George[US/US]; US
ROWE, Alexander McIntyre[US/US]; US
UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION[US/US]; 1st Floor Gardner Steel Conference Center 130 Thackeray Avenue Pittsburgh, Pennsylvania 15260, US
Inventors: SHLOMCHIK, Mark; US
PANOUSIS, Constantinos George; US
ROWE, Alexander McIntyre; US
TURQUETI NEVES, Adriana; DE
SCHITTLER NEVES, Eduardo; DE
Agent: FINN, J., Patrick, III; US
Priority Data:
62/427,33529.11.2016US
Title (EN) METHODS AND MATERIALS FOR CLONING FUNCTIONAL T CELL RECEPTORS FROM SINGLE T CELLS
(FR) MÉTHODES ET MATÉRIELS DE CLONAGE DE RÉCEPTEURS DE LYMPHOCYTES T FONCTIONNELS À PARTIR DE LYMPHOCYTES T UNIQUES
Abstract:
(EN) This document provides methods and materials involved in cloning functional TCRs from single T cells. Methods and materials for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, kits for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, methods for making such kits, collections of nucleic acid primers designed to amplify the entire coding sequence of both variable regions for each expressed V segment for functional.alpha. beta, or.gamma.delta. TCRs of a particular mammalian species, methods for using such collections of nucleic acid primers to clone functional TCRs from single T cells, and kits containing such collections of nucleic acid primers to clone functional TC Rs from single T cells are provided.
(FR) La présente invention concerne des méthodes et des matériels intervenant dans le clonage de TCR fonctionnels à partir de lymphocytes T uniques. Par exemple, l'invention concerne des méthodes et des matériels permettant d'obtenir un acide nucléique codant un TCR à partir d'un lymphocyte T unique et d'agencer cet acide nucléique pour former des vecteurs d'acide nucléique conçus avec succès pour exprimer un TCR, des kits permettant d'obtenir un acide nucléique codant un TCR à partir d'un lymphocyte T unique et d'agencer cet acide nucléique pour former des vecteurs d'acide nucléique conçus avec succès pour exprimer un TCR, des procédés de fabrication de tels kits, des collections d'amorces d'acide nucléique conçues pour amplifier toute la séquence de codage des deux régions variables pour chaque segment V exprimé pour des TCR fonctionnels αβ ou γδ d'une espèce de mammifère particulière, des méthodes d'utilisation de telles collections d'amorces d'acide nucléique pour cloner des TCR fonctionnels à partir de lymphocytes T uniques, et des kits contenant de telles collections d'amorces d'acide nucléique pour cloner des TCR fonctionnels à partir de lymphocytes T uniques.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)