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1. (WO2018102411) METHODS AND APPARATUS FOR SIMULTANEOUSLY DETECTING A LARGE RANGE OF PROTEIN CONCENTRATIONS
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Pub. No.: WO/2018/102411 International Application No.: PCT/US2017/063704
Publication Date: 07.06.2018 International Filing Date: 29.11.2017
IPC:
B01D 15/00 (2006.01) ,C07K 1/26 (2006.01) ,C07K 1/28 (2006.01) ,G01N 1/20 (2006.01) ,G01N 27/00 (2006.01) ,G01N 27/447 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
24
by electrochemical means
26
Electrophoresis
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
24
by electrochemical means
26
Electrophoresis
28
Isoelectric focusing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
02
Devices for withdrawing samples
10
in the liquid or fluent state
20
for flowing or falling materials
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
416
Systems
447
using electrophoresis
Applicants:
PROTEINSIMPLE [US/US]; 3001 Orchard Parkway San Jose, CA 95134, US
Inventors:
XU, Hui; US
SWARTZ, Kenneth; US
Agent:
MILCH, Erik, B.; US
FERNSTROM, Anders E.; US
HUTTER, Christopher R.; US
Priority Data:
62/427,62429.11.2016US
Title (EN) METHODS AND APPARATUS FOR SIMULTANEOUSLY DETECTING A LARGE RANGE OF PROTEIN CONCENTRATIONS
(FR) PROCÉDÉS ET APPAREIL POUR DÉTECTER SIMULTANÉMENT UNE LARGE PLAGE DE CONCENTRATIONS DE PROTÉINES
Abstract:
(EN) Some embodiments described herein relate to a method that includes separating an analyte- containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP- conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.
(FR) Certains modes de réalisation de la présente invention concernent un procédé qui comprend la séparation d'un échantillon contenant un analyte par électrophorèse dans un capillaire. Le capillaire est chargé avec un agent de chimioluminescence, tel que le luminol, qui est configuré pour réagir avec l'analyte (par exemple, des protéines conjuguées à HRP) pour produire un signal indicatif d'une concentration et/ou d'une quantité d'analyte à chaque emplacement le long de la longueur du capillaire. Une première image du capillaire contenant les analytes et l'agent de chimioluminescence est capturée sur une première période de temps. Une seconde image du capillaire contenant les analytes et l'agent de chimioluminescence est capturée sur une seconde période de temps plus longue. Une concentration et/ou une quantité d'une première population d'analytes à un premier emplacement est déterminée à l'aide de la première image, et une concentration et/ou une quantité d'une seconde population d'analytes à un second emplacement est déterminée à l'aide de la seconde image.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)