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1. (WO2018102201) IN VITRO EPITHELIAL MODELS COMPRISING LAMINA PROPRIA-DERIVED CELLS

Pub. No.:    WO/2018/102201    International Application No.:    PCT/US2017/062817
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Wed Nov 22 00:59:59 CET 2017
IPC: C12M 3/00
C12M 3/04
C12M 1/34
G01N 33/50
Applicants: EMULATE, INC.
Inventors: LEVNER, Daniel
KERNS, S. Jordan
BARRILE, Riccardo
HAMILTON, Geraldine
KARALIS, Catherine
LEVNER, Daniel
LUCCHESI, Carolina
VARONE, Antonio
VILLENAVE, Remi
Title: IN VITRO EPITHELIAL MODELS COMPRISING LAMINA PROPRIA-DERIVED CELLS
Abstract:
An in vitro microfluidic "organ-on-chip" is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic "organ-on-chip", e.g. "epithelia-on-chip" further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic "organ-on-chips" allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.