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1. (WO2018101861) DEVICE FOR MONITORING THE SPATIAL AND TEMPORAL DYNAMICS OF THROMBIN

Pub. No.:    WO/2018/101861    International Application No.:    PCT/RU2017/050116
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Mon Nov 13 00:59:59 CET 2017
IPC: G01N 33/86
G01N 21/64
G01N 33/52
Applicants: OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTYU «HEMACORE LABS»
ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "ГЕМАКОР ЛАБС"
Inventors: KARAMZIN, Sergey Sergeevich
КАРАМЗИН, Сергей Сергеевич
ATAULLAKHANOV, Fazoil Inoyatovich
АТАУЛЛАХАНОВ, Фазоил Иноятович
NABIRKIN, Aleksey Nikolaevich
НАБИРКИН, Алексей Николаевич
DASHKEVICH, Nataljya Mikhajlovna
ДАШКЕВИЧ, Наталья Михайловна
Title: DEVICE FOR MONITORING THE SPATIAL AND TEMPORAL DYNAMICS OF THROMBIN
Abstract:
A device for monitoring the spatial and temporal dynamics of thrombin, comprising a temperature-controlled sealed chamber with a transparent window and a light trap, said chamber being filled with a fluid medium and being designed to be capable of accommodating a cuvette containing a study sample of blood plasma, and into which a clot activating insert is introduced having a substance which initiates the clotting process applied to the lower end thereof, at least one means for lighting the sample, which is designed to be capable of receiving a light scattering signal from the sample, and at least one first irradiating means designed to be capable of exciting a fluorescence signal of a special marker which forms in the sample during the cleavage of a fluorogenic substrate, pre-added to the sample, by one of the proteolytic enzymes of the clotting system, a means for optically photo/video recording light scattering/radiation from the sample, and a means for adjusting the pressure in the temperature-controlled sealed chamber, which is designed to be capable of maintaining a pressure in the chamber which is greater than atmospheric pressure, wherein the device comprises at least one second means for irradiating the sample, designed to be capable of exciting a fluorescence signal of the said marker, and wherein the at least one first irradiating means allows the sample to be irradiated in a direction perpendicular to the plane of the cuvette, and the at least one second irradiating means allows the sample to be irradiated at an angle to the plane of the cuvette.