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1. (WO2018099719) ALCOHOL ACETYL TRANSFERASES FOR ALKYL ALKANOATE PRODUCTION

Pub. No.:    WO/2018/099719    International Application No.:    PCT/EP2017/079111
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Wed Nov 15 00:59:59 CET 2017
IPC: C12P 7/62
C12N 9/10
Applicants: WAGENINGEN UNIVERSITEIT
Inventors: KRUIS, Aleksander Johannes
LEVISSON, Mark
MARS, Astrid Elisabeth
KENGEN, Servatius Wilhelmus Maria
VAN DER OOST, John
SANDERS, Johan Pieter Marinus
WEUSTHUIS, Ruud Alexander
Title: ALCOHOL ACETYL TRANSFERASES FOR ALKYL ALKANOATE PRODUCTION
Abstract:
The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.