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1. (WO2018099479) HIGH-PURITY D-PSICOSE PREPARATION METHOD

Pub. No.:    WO/2018/099479    International Application No.:    PCT/CN2017/114289
Publication Date: Fri Jun 08 01:59:59 CEST 2018 International Filing Date: Sat Dec 02 00:59:59 CET 2017
IPC: C12N 9/90
C12P 19/24
C12P 19/02
Applicants: SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
山东百龙创园生物科技股份有限公司
Inventors: DOU, Baode
窦宝德
DOU, Guangpeng
窦光朋
ZHANG, Mingzhan
张明站
SHAO, Xianbao
邵先豹
LI, Fanghua
李方华
DU, Qian
杜倩
YANG, Tengteng
杨腾腾
Title: HIGH-PURITY D-PSICOSE PREPARATION METHOD
Abstract:
A preparation method for high purity D-psicose, having the following steps: (1) after centrifuging a fermentation broth of Bacillus subtilis, extracted bacteria going through homogenization processing, obtaining a mixed liquor containing D-psicose 3-epimerase; (2) formulating a fructose solution and adding the solution to the mixed liquor containing D-psicose 3-epimerase, adjusting pH value, adding cobalt chloride, performing a preserved-heat reaction, then feeding fructose solution into the reaction liquid, continuing the reaction, and stopping the reaction, obtaining crude D-psicose liquid; (3) after performing decoloring, filtering, ion exchange, chromatographic separation and concentration on the crude D-psicose liquid, crystallizing or drying the liquid, obtaining D-psicose.