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|1. (WO2018099479) HIGH-PURITY D-PSICOSE PREPARATION METHOD|
|Applicants:||SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
|Title:||HIGH-PURITY D-PSICOSE PREPARATION METHOD|
A preparation method for high purity D-psicose, having the following steps: (1) after centrifuging a fermentation broth of Bacillus subtilis, extracted bacteria going through homogenization processing, obtaining a mixed liquor containing D-psicose 3-epimerase; (2) formulating a fructose solution and adding the solution to the mixed liquor containing D-psicose 3-epimerase, adjusting pH value, adding cobalt chloride, performing a preserved-heat reaction, then feeding fructose solution into the reaction liquid, continuing the reaction, and stopping the reaction, obtaining crude D-psicose liquid; (3) after performing decoloring, filtering, ion exchange, chromatographic separation and concentration on the crude D-psicose liquid, crystallizing or drying the liquid, obtaining D-psicose.