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1. (WO2018098935) VECTOR FOR PLANT GENOME SITE-DIRECTED BASE SUBSTITUTION
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Pub. No.: WO/2018/098935 International Application No.: PCT/CN2017/077133
Publication Date: 07.06.2018 International Filing Date: 17.03.2017
IPC:
C12N 15/82 (2006.01) ,C12N 9/78 (2006.01) ,A01H 5/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
78
acting on carbon to nitrogen bonds other than peptide bonds (3.5)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
Applicants:
中国科学院上海生命科学研究院 SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES [CN/CN]; 中国上海市 徐汇区岳阳路319号 319 Yueyang Road, Xuhui District Shanghai 200031, CN
Inventors:
朱健康 ZHU, Jiankang; CN
陆钰明 LU, Yuming; CN
Agent:
上海一平知识产权代理有限公司 XU & PARTNERS, LLC.; 中国上海市 普陀区真北路958号天地科技广场1号楼106室 Room No.106, Building No.1, Universal High-Tech Plaza,958 Zhen Bei Road, Putuo District Shanghai 200333, CN
Priority Data:
201611180376.002.12.2016CN
Title (EN) VECTOR FOR PLANT GENOME SITE-DIRECTED BASE SUBSTITUTION
(FR) VECTEUR POUR SUBSTITUTION DE BASE DIRIGÉE DU GÉNOME D'UNE PLANTE
(ZH) 一种用于植物基因组定点碱基替换的载体
Abstract:
(EN) Disclosed are a nucleic acid construct having a particular structure, and a method for a base mutation or a base substitution on a particular site of a plant genome using the construct. The nucleic acid construct comprises a first expression cassette, a second expression cassette and an optional third expression cassette, wherein the first expression cassette is an expression cassette for expressing gRNAs, the second expression cassette is an expression cassette for expressing a fusion protein comprising a CAS9 and cytosine deaminase, and the third expression cassette is an expression cassette for expressing a selection marker.
(FR) L'invention concerne une construction d'acide nucléique ayant une structure particulière, et un procédé pour une mutation de base ou une substitution de base sur un site particulier d'un génome de plante à l'aide de la construction. La construction d'acide nucléique comprend une première cassette d'expression, une deuxième cassette d'expression et une troisième cassette d'expression facultative, la première cassette d'expression étant une cassette d'expression pour exprimer des ARNg, la deuxième cassette d'expression étant une cassette d'expression pour exprimer une protéine de fusion comprenant une CAS9 et une cytosine désaminase, et la troisième cassette d'expression étant une cassette d'expression pour exprimer un marqueur de sélection.
(ZH) 一种具有特定结构的核酸构建物,以及利用该构建物在植物基因组的特定位点进行碱基突变或碱基替换的方法。所述核酸构建物包括第一表达盒,第二表达盒以及任选的第三表达盒,其中第一表达盒为用于表达gRNA的表达盒,第二表达盒为用于表达包含CAS9和胞嘧啶脱氨酶的融合蛋白的表达盒,第三表达盒为用于表达筛选标记的表达盒。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)