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1. (WO2018098671) A METHOD FOR CRISPR LIBRARY SCREENING
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Pub. No.: WO/2018/098671 International Application No.: PCT/CN2016/107952
Publication Date: 07.06.2018 International Filing Date: 30.11.2016
IPC:
C40B 40/08 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/85 (2006.01)
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40
Libraries per se, e.g. arrays, mixtures
04
Libraries containing only organic compounds
06
Libraries containing nucleotides or polynucleotides, or derivatives thereof
08
Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
Applicants:
CHINA AGRICULTURAL UNIVERSITY [CN/CN]; State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University Beijing 100193, CN
Inventors:
WU, Sen; CN
XU, Chunlong; CN
QI, Xiaolan; CN
DU, Xuguang; CN
ZOU, Huiying; CN
Agent:
F & S INTELLECTUAL PROPERTY LTD.; Room 1402, Tower B, Horizon International Tower, No. 6 Zhichun Road, Haidian District Beijing 100191, CN
Priority Data:
Title (EN) A METHOD FOR CRISPR LIBRARY SCREENING
(FR) PROCÉDÉ DE CRIBLAGE DE BIBLIOTHÈQUE DE CRISPR
Abstract:
(EN) Provided is a genome library comprising a plurality of PB-mediated CRISPR system polynucleotide which comprises minimal guide RNAs flanked by minimal piggybac inverted repeat elements. A method of in vivo genome-scale screening using the said polynucleotide library is also provided.
(FR) L'invention concerne une bibliothèque génomique comprenant une pluralité de polynucléotides de système CRISPR à médiation par PB comprenant des ARN guides minimaux flanqués d'éléments de répétitions inversées de PiggyBac minimaux. L'invention concerne également un procédé de criblage in vivo à l'échelle du génome, mettant en oeuvre ladite bibliothèque de polynucléotides.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)