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1. (WO2018089865) MICROSCOPY DEVICES, METHODS AND SYSTEMS

Pub. No.:    WO/2018/089865    International Application No.:    PCT/US2017/061205
Publication Date: Fri May 18 01:59:59 CEST 2018 International Filing Date: Mon Nov 13 00:59:59 CET 2017
IPC: G02B 21/00
G01N 21/64
G02B 21/36
Applicants: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors: HILLMAN, Elizabeth, M.C.
Title: MICROSCOPY DEVICES, METHODS AND SYSTEMS
Abstract:
A pulsed beam of NIR excitation light is projected into a sample (345) at an oblique angle and scanned by a scanning element through a volume in the sample. 2-photon excitation excites fluorescence within the sample. The fluorescence is imaged onto an intermediate image plane that remains stationary regardless of the orientation of the scanning element. The image is captured by a linear array of light detecting elements (392) or a linear portion of a rectangular array. At any given position of the scanning element, the linear array (or portion) images all depths simultaneously. A plurality of images are captured for each of a plurality of different orientations of the scanning element. The orientation of the scanning element is controlled to move in a two dimensional pattern, which causes the beam of excitation light to sweep out a three dimensional volume within the sample.