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1. (WO2018089550) METHODS OF PRODUCING AMPLIFIED DOUBLE STRANDED DEOXYRIBONUCLEIC ACIDS AND COMPOSITIONS AND KITS FOR USE THEREIN
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Pub. No.: WO/2018/089550 International Application No.: PCT/US2017/060717
Publication Date: 17.05.2018 International Filing Date: 08.11.2017
IPC:
C12P 19/34 (2006.01) ,C12Q 1/6806 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
[IPC code unknown for C12Q 1/6806]
Applicants:
TAKARA BIO USA, INC. [US/US]; 1290 Terra Bella Avenue Mountain View, California 94043, US
Inventors:
TORI, Kazuo; US
BOSTICK, Magnolia; US
FARMER, Andrew; US
Agent:
FIELD, Bret E.; US
Priority Data:
62/420,50310.11.2016US
Title (EN) METHODS OF PRODUCING AMPLIFIED DOUBLE STRANDED DEOXYRIBONUCLEIC ACIDS AND COMPOSITIONS AND KITS FOR USE THEREIN
(FR) PROCÉDÉS DE PRODUCTION D'ACIDES DÉSOXYRIBONUCLÉIQUES DOUBLE BRIN AMPLIFIÉS ET COMPOSITIONS ET KITS DESTINÉS À ÊTRE UTILISÉS DANS CEUX-CI
Abstract:
(EN) Methods of producing an amplified double stranded deoxyribonucleic acid (dsDNA) from a nucleic acid sample are provided. Aspects of the methods include amplifying using a single product nucleic acid primer and a template switch oligonucleotide to produce an amplified dsDNA product. Compositions and kits for use in performing the methods are also provided.
(FR) L'invention concerne des procédés de production d'un acide désoxyribonucléique double brin amplifié (ADNdb) à partir d'un échantillon d'acide nucléique. Des aspects des procédés comprennent l'amplification à l'aide d'une amorce d'acide nucléique de produit unique et d'un oligonucléotide de commutation de matrice pour produire un produit d'ADNdb amplifié. L’invention concerne en outre des compositions et des kits pour mettre en œuvre les procédés.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)