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1. (WO2018089438) MULTIPLEXED IMAGING USING MERFISH, EXPANSION MICROSCOPY, AND RELATED TECHNOLOGIES
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/089438 International Application No.: PCT/US2017/060558
Publication Date: 17.05.2018 International Filing Date: 08.11.2017
IPC:
G01N 1/36 (2006.01) ,C12Q 1/68 (2018.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
36
Embedding or analogous mounting of samples
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE[US/US]; 17 Quincy Street Cambridge, MA 02138, US
Inventors: ZHUANG, Xiaowei; US
MOFFITT, Jeffrey, R.; US
WANG, Guiping; US
Agent: CHEN, Tani; US
Priority Data:
62/419,03308.11.2016US
62/569,12706.10.2017US
Title (EN) MULTIPLEXED IMAGING USING MERFISH, EXPANSION MICROSCOPY, AND RELATED TECHNOLOGIES
(FR) IMAGERIE MULTIPLEXÉE UTILISANT LA TECHNIQUE MERFISH, LA MICROSCOPIE À EXPANSION ET LES TECHNOLOGIES ASSOCIÉES
Abstract:
(EN) The present invention generally relates to microscopy, and to systems and methods for imaging or determining nucleic acids or other desired targets, for instance, within cells. In certain aspects, a sample is contained within an expandable material, which is expanded and imaged in some fashion. Expansion of the material improves the effective resolution of the subsequent image. This may be combined, for example, with other super-resolution techniques, such as STORM, and/or with techniques such as MERFISH for determining nucleic acids such as mRNA within the sample, for example, by binding nucleic acid probes to the sample. Other aspects are generally directed to compositions or devices for use in such methods, kits for use in such methods, or the like.
(FR) La présente invention concerne de manière générale la microscopie, et des systèmes et des procédés d'imagerie ou de détermination d'acides nucléiques ou d'autres cibles souhaitées, par exemple, à l'intérieur de cellules. Dans certains aspects, un échantillon est contenu dans un matériau expansible, qui est expansé et imagé d'une certaine manière. L'expansion du matériau améliore la résolution efficace de l'image suivante. Ceci peut être combiné, par exemple, avec d'autres techniques de super-résolution, telles que STORM, et/ou avec des techniques telles que MERFISH pour déterminer des acides nucléiques tels que l'ARNm dans l'échantillon, par exemple, par liaison de sondes d'acide nucléique à l'échantillon. D'autres aspects concernent généralement des compositions ou des dispositifs destinés à être utilisés dans de tels procédés, des kits destinés à être utilisés dans de tels procédés, ou analogues.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)