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1. (WO2018087312) ILLUMINATION ARRANGEMENT AND METHOD FOR ILLUMINATION IN A MICROSCOPE AND MICROSCOPE

Pub. No.:    WO/2018/087312    International Application No.:    PCT/EP2017/078929
Publication Date: Fri May 18 01:59:59 CEST 2018 International Filing Date: Sat Nov 11 00:59:59 CET 2017
IPC: G02B 21/00
G02B 21/06
Applicants: LEICA MICROSYSTEMS CMS GMBH
Inventors: FAHRBACH, Florian
Title: ILLUMINATION ARRANGEMENT AND METHOD FOR ILLUMINATION IN A MICROSCOPE AND MICROSCOPE
Abstract:
The invention relates to an illumination arrangement (3) for a microscope (1a), in particular a light sheet or SPIM microscope (1) or an inclined plane microscope such as an OPM or SCAPE microscope, comprising an illumination entrance (13) for admitting an illuminating beam (7) and an illumination exit (15) for emitting at least two partial beams (17) generated from the illuminating beam (7), as well as a microscope (1a) having such an illumination arrangement (3) and a method for uniformly illuminating a sample (63) in a microscope (1a). Illumination arrangements (3) and microscopes (1a) from the prior art are often susceptible to wear and are limited in terms of illumination frequencies. In order to eliminate these disadvantages, the illumination arrangement (3) comprises at least one diffractive optical component (46) for dividing the illuminating beam (7) into the at least two partial beams (17) propagating along partial beam paths (19) and for relatively changing the propagation directions (11) of the at least two partial beams (17).