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1. (WO2018087301) METHODS FOR IMPROVING DROUGHT RESISTANCE IN PLANTS - KGDR06, KGDR26, KGDR25, KGDR42 AND KGDR37

Pub. No.:    WO/2018/087301    International Application No.:    PCT/EP2017/078909
Publication Date: Fri May 18 01:59:59 CEST 2018 International Filing Date: Sat Nov 11 00:59:59 CET 2017
IPC: C07K 14/415
C12N 9/14
C12N 15/82
Applicants: KEYGENE N.V.
Inventors: DESLATTES MAYS, Anne
MUNKVOLD, Jesse David
DILEO, Matthew Vitabile
Title: METHODS FOR IMPROVING DROUGHT RESISTANCE IN PLANTS - KGDR06, KGDR26, KGDR25, KGDR42 AND KGDR37
Abstract:
Disclosed here is a method for improving drought resistance in plants, comprising overexpressing or expressing de novo a KGDR06 protein, a KGDR26 protein, a KGDR25 protein, a KGDR42 protein, a KGDR37 protein, or any combination thereof, in a plant, plant protoplast or plant cell, wherein the KGDR06 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25-27, 29, 31, 33, 35, 37, 39-43, 45, 47, 49, 51, and 53, wherein the KGDR26 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87 and 89, wherein the KGDR25 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 91, 93, 95, 97, 99-100, 102, 104, 106-107, 109, 111-113 and 115, wherein the KGDR42 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 117, 119, 121, 123 and 125, and wherein the KGDR37 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168. Also described is a plant, plant protoplast or plant cell having improved drought resistance, wherein the plant, plant protoplast or plant cell has been genetically engineered to overexpress or express de novo a KGDR06 protein that comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25-27, 29, 31, 33, 35, 37, 39-43, 45, 47, 49, 51, and 53, a KGDR26 protein that comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87 and 89, a KGDR25 protein that comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 91, 93, 95, 97, 99-100, 102, 104, 106-107, 109, 11-113 and 115, a KGDR42 protein that comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 117, 119, 121, 123 and 125, a KGDR37 protein that comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168, or any combination thereof.