Search International and National Patent Collections

1. (WO2018085584) A METHOD OF DETECTING NUCLEOTIDE POLYMORPHISM ASSOCIATED WITH RISK OF DEVELOPING PSYCHOSIS AND DETERMINING INDIVIDUAL GENOTYPE

Pub. No.:    WO/2018/085584    International Application No.:    PCT/US2017/059784
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Fri Nov 03 00:59:59 CET 2017
IPC: A61K 31/7088
C12N 15/09
C12Q 1/68
G01N 33/50
Applicants: VITALQ, INC.
Inventors: CHAN, Perry
Title: A METHOD OF DETECTING NUCLEOTIDE POLYMORPHISM ASSOCIATED WITH RISK OF DEVELOPING PSYCHOSIS AND DETERMINING INDIVIDUAL GENOTYPE
Abstract:
A method for conducting an allele- specific real time polymerase chain reaction to detect a nucleotide polymorphism, NC_000014.8:g.105239192T>C, of the v-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) gene including the steps: a. obtaining a genomic DNA sample from a test subject; b. subjecting said genomic DNA sample to two separate polymerase chain reactions; c.utilizing in said one of the polymerase chain reactions with a primer specific to direct amplification of the T-allele with the following sequence: SEQ1: T- Allele 5 ' -GGGGATGGAGGTGTAGCCCGT-3 '; d. utilizing in said the other polymerase chain reactions with a primer specific to direct amplification of the C-allele with the following sequence: SEQ2: C-Allele: 5 ' -GGGGATGGAGGTGTAGCCCGC-3 '; e. utilizing in said both polymerase chain reactions with a primer to direct amplification of either alleles with the following sequence: SEQ3: 5'- GCGGTCGCCTGCCCTTCTACAACC-3'; and f. utilizing a composition for amplifying and monitoring a target nucleic acid sequence in the sample.