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1. (WO2018085572) METHOD AND APPARATUS FOR SELECTIVE REMOVAL OF CELLS FROM A CELL SUSPENSION BY MECHANICAL LYSIS

Pub. No.:    WO/2018/085572    International Application No.:    PCT/US2017/059768
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Fri Nov 03 00:59:59 CET 2017
IPC: C12Q 1/02
C12Q 1/04
G01N 1/40
G01N 33/487
Applicants: UNIVERSITY OF IOWA RESEARCH FOUNDATION
HENRY, Michael, D.
KROG, Benjamin
VIGMOSTAD, Sarah
CABLE, Mike
Inventors: HENRY, Michael, D.
KROG, Benjamin
VIGMOSTAD, Sarah
CABLE, Mike
Title: METHOD AND APPARATUS FOR SELECTIVE REMOVAL OF CELLS FROM A CELL SUSPENSION BY MECHANICAL LYSIS
Abstract:
In some embodiments, the present disclosure provides an apparatus and methods for selectively lysing cells from a cell suspension including disassociating a population of cells from a tissue sample, the population of cells including a population of one or more viable cells and a population of one or more dead and dying cells, exposing the population of cells to at least one pulse of fluid shear stress having a force along a conduit wall of from about 500 dyn/cm2 to about 2500 dyn/cm2 to substantially lyse the population of one or more dead or dying cells, leaving the population of one or more viable cells substantially intact, and collecting the population of one or more viable cells.