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1. (WO2018085572) METHOD AND APPARATUS FOR SELECTIVE REMOVAL OF CELLS FROM A CELL SUSPENSION BY MECHANICAL LYSIS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/085572 International Application No.: PCT/US2017/059768
Publication Date: 11.05.2018 International Filing Date: 02.11.2017
IPC:
C12Q 1/02 (2006.01) ,C12Q 1/04 (2006.01) ,G01N 1/40 (2006.01) ,G01N 33/487 (2006.01)
Applicants: HENRY, Michael, D.[US/US]; US
KROG, Benjamin[US/US]; US
VIGMOSTAD, Sarah[US/US]; US
CABLE, Mike[US/US]; US
UNIVERSITY OF IOWA RESEARCH FOUNDATION[US/US]; 112 N. Capitol Street 6 Gilmore Hall Iowa City, Iowa 52242-5500, US
Inventors: HENRY, Michael, D.; US
KROG, Benjamin; US
VIGMOSTAD, Sarah; US
CABLE, Mike; US
Agent: VIKSNINS, Ann, S.; US
HARRIS, Robert, J.; US
MALEN, Peter, L.; US
KRUEGER, Katherine, A.D.; US
MICKELSON, John, W.; US
PADYS, Danny, J.; US
ANDRUS, Alex; US
Priority Data:
62/416,60902.11.2016US
Title (EN) METHOD AND APPARATUS FOR SELECTIVE REMOVAL OF CELLS FROM A CELL SUSPENSION BY MECHANICAL LYSIS
(FR) PROCÉDÉ ET APPAREIL D'ÉLIMINATION SÉLECTIVE DE CELLULES D'UNE SUSPENSION CELLULAIRE PAR LYSE MÉCANIQUE
Abstract: front page image
(EN) In some embodiments, the present disclosure provides an apparatus and methods for selectively lysing cells from a cell suspension including disassociating a population of cells from a tissue sample, the population of cells including a population of one or more viable cells and a population of one or more dead and dying cells, exposing the population of cells to at least one pulse of fluid shear stress having a force along a conduit wall of from about 500 dyn/cm2 to about 2500 dyn/cm2 to substantially lyse the population of one or more dead or dying cells, leaving the population of one or more viable cells substantially intact, and collecting the population of one or more viable cells.
(FR) La présente invention concerne, dans certains modes de réalisation, un appareil et des procédés de lyse sélective de cellules à partir d'une suspension cellulaire, le procédé comprenant la dissociation d'une population de cellules à partir d'un échantillon de tissu, la population de cellules comprenant une population d'une ou plusieurs cellules viables et une population d'une ou de plusieurs cellules mortes et mourantes, l'exposition de la population de cellules à au moins une impulsion de contrainte de cisaillement de fluide ayant une force le long d'une paroi de conduit d'environ 500 dyn/cm2 à environ 2500 dyn/cm2 pour lyser sensiblement la population d'une ou de plusieurs cellules mortes ou mourantes, la sortie de la population d'une ou de plusieurs cellules viables sensiblement intactes, et la collecte de la population d'une ou de plusieurs cellules viables.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)