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1. (WO2018085419) METHOD OF PRODUCING NAIVE PLURIPOTENT STEM CELLS

Pub. No.:    WO/2018/085419    International Application No.:    PCT/US2017/059555
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Thu Nov 02 00:59:59 CET 2017
IPC: C12N 5/00
C12N 5/16
Applicants: FENG, Jian
Inventors: FENG, Jian
Title: METHOD OF PRODUCING NAIVE PLURIPOTENT STEM CELLS
Abstract:
Provided herein are compositions and methods for generation of naive human pluripotent stem cells. The method comprises incubation of iPSCs under 5% O2 in a medium comprising 5% glucose, an MEK inhibitor, a GSK3β inhibitor, human leukemia inhibitory factor (LIF), human insulin and Torin 1. The method does not need any other inhibitors or transgene expression. The naive human pluripotent cells can be used to generate a large amount of mature human cells from all three germ layers in host non-human animals.