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1. (WO2018084271) FLUORESCENCE OBSERVATION DEVICE

Pub. No.:    WO/2018/084271    International Application No.:    PCT/JP2017/039835
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Fri Nov 03 00:59:59 CET 2017
IPC: G01N 21/64
G02B 21/06
G02B 26/10
Applicants: NIKON CORPORATION
株式会社ニコン
Inventors: DAKE Fumihiro
嶽 文宏
Title: FLUORESCENCE OBSERVATION DEVICE
Abstract:
A fluorescence observation device according to the present invention is provided with: a first intensity modulation unit for modulating the intensity of pump light at a frequency f1, the pump light exciting fluorescence in an observation object; a second intensity modulation unit for modulating the intensity of probe light at a frequency f2 different from the frequency f1, the probe light inducing stimulated emission by the observation object; a light receiving unit for receiving fluorescence from the observation object irradiated by the intensity-modulated pump light and probe light; and a detection unit for detecting a frequency i × f1±j × f2 (where i and j are positive integers, and i and/or j is 2 or greater) component in a light reception signal detected by the light receiving unit.