Search International and National Patent Collections

1. (WO2018083308) MASS SPECTROMETRY-BASED METHODS FOR THE DETECTION OF CIRCULATING HISTONES H3 AND H2B IN PLASMA FROM SEPSIS OR SEPTIC SHOCK (SS) PATIENTS

Pub. No.:    WO/2018/083308    International Application No.:    PCT/EP2017/078362
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Tue Nov 07 00:59:59 CET 2017
IPC: G01N 33/68
Applicants: CENTRO DE INVESTIGACIÓN BIOMÉDICA EN RED (CIBER-ISCIII)
FUNDACIÓN INCLIVA
UNIVERSITAT DE VALÈNCIA ESTUDI GENERAL
Inventors: GARCÍA GIMÉNEZ, José, Luis
ROMÁ MATEO, Carlos
PALLARDÓ CALATAYUD, Federico, V.
Title: MASS SPECTROMETRY-BASED METHODS FOR THE DETECTION OF CIRCULATING HISTONES H3 AND H2B IN PLASMA FROM SEPSIS OR SEPTIC SHOCK (SS) PATIENTS
Abstract:
The present invention proposes a novel method for the detection of circulating histones H3 and H2B in plasma from sepsis or septic shock (SS) patients, based on mass spectrometry-based methods, in particular based on multiple reaction monitoring targeted mass spectrometry (MRM-MS). Such methods allow quantification of histones using an internal standard and show strong specificity and sensitivity values.