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|1. (WO2018082143) DNA TETRAHEDRON MODIFIED BY APTAMER AS1411 AND PREPARATION METHOD THEREFOR|
|Title:||DNA TETRAHEDRON MODIFIED BY APTAMER AS1411 AND PREPARATION METHOD THEREFOR|
Provided are a DNA tetrahedron modified by aptamer AS1411. The preparation method comprises the following steps: (1) connecting an AS1411 sequence to the 5 'end of any one of DNA single chains of a DNA tetrahedron, and dissolving the obtained DNA powder by means DNA synthesis; (2) determining the absorbance of DNA at the wavelength of 260 nm and 330 nm, and then calculating the total volume of four single strands; and (3) according to the calculation results in step (2), absorbing the DNA obtained in the step (1) and mixing DNA with TM-buffer, vibrating by vortex and uniformly mixing to place in a PCR instrument, rapidly heating to 95ºC and stabilizing for 10 min, then cooling to 4ºC and stabilizing for 20 min, and finally storing at minus 20ºC.