Search International and National Patent Collections

1. (WO2018082143) DNA TETRAHEDRON MODIFIED BY APTAMER AS1411 AND PREPARATION METHOD THEREFOR

Pub. No.:    WO/2018/082143    International Application No.:    PCT/CN2016/107816
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Thu Dec 01 00:59:59 CET 2016
IPC: C12N 15/10
C12N 15/115
C12N 15/11
Applicants: SICHUAN UNIVERSITY
四川大学
Inventors: LIN, Yunfeng
林云锋
LI, Qianshun
李谦顺
CAI, Xiaoxiao
蔡潇潇
LIN, Shiyu
林世宇
SHAO, Xiaoru
邵晓茹
Title: DNA TETRAHEDRON MODIFIED BY APTAMER AS1411 AND PREPARATION METHOD THEREFOR
Abstract:
Provided are a DNA tetrahedron modified by aptamer AS1411. The preparation method comprises the following steps: (1) connecting an AS1411 sequence to the 5 'end of any one of DNA single chains of a DNA tetrahedron, and dissolving the obtained DNA powder by means DNA synthesis; (2) determining the absorbance of DNA at the wavelength of 260 nm and 330 nm, and then calculating the total volume of four single strands; and (3) according to the calculation results in step (2), absorbing the DNA obtained in the step (1) and mixing DNA with TM-buffer, vibrating by vortex and uniformly mixing to place in a PCR instrument, rapidly heating to 95ºC and stabilizing for 10 min, then cooling to 4ºC and stabilizing for 20 min, and finally storing at minus 20ºC.