Search International and National Patent Collections

1. (WO2018082093) ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM

Pub. No.:    WO/2018/082093    International Application No.:    PCT/CN2016/104882
Publication Date: Sat May 12 01:59:59 CEST 2018 International Filing Date: Tue Nov 08 00:59:59 CET 2016
IPC: C12N 7/01
C12N 15/66
C12N 15/65
Applicants: WUHAN INSTITUTE OF VIROLOGY, CHINESE ACADEMY OF SCIENCES
Inventors: LUO, Minhua
ZENG, Wenbo
JIANG, Haifei
ZHAO, Fei
YANG, Hong
SONG, Yige
SHEN, Zhangzhou
Title: ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM
Abstract:
An anterograde monosynaptic transneuronal viral tracer system for mapping the direct postsynaptic targets of specific neuron types in a given brain nucleus comprises a H129-derived recombinant defective HSV-1 virus comprising an integrated first expression cassette that contains a first promoter, a first fluorescent protein-encoding sequence, and a resistance peptide-encoding sequence, wherein the first expression cassette replaces the whole or partial sequence of thymidine kinase (TK) gene so that the H129-derived recombinant defective HSV-1 virus has a loss of TK function; and an AAV9-derived recombinant AAV9 helper virus comprising an integrated second expression cassette that contains a second promoter, a TK-encoding sequence, a linker peptide-encoding sequence, and a second fluorescent protein-encoding sequence, where the expression of TK from the second expression cassette enables the H129-derived recombinant defective HSV-1 virus to replicate; wherein the first and second fluorescent protein-encoding sequences encode different fluorescent proteins.