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|1. (WO2018082093) ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM|
|Applicants:||WUHAN INSTITUTE OF VIROLOGY, CHINESE ACADEMY OF SCIENCES
|Title:||ANTEROGRADE MONOSYNAPTIC TRANSNEURONAL TRACER SYSTEM|
An anterograde monosynaptic transneuronal viral tracer system for mapping the direct postsynaptic targets of specific neuron types in a given brain nucleus comprises a H129-derived recombinant defective HSV-1 virus comprising an integrated first expression cassette that contains a first promoter, a first fluorescent protein-encoding sequence, and a resistance peptide-encoding sequence, wherein the first expression cassette replaces the whole or partial sequence of thymidine kinase (TK) gene so that the H129-derived recombinant defective HSV-1 virus has a loss of TK function; and an AAV9-derived recombinant AAV9 helper virus comprising an integrated second expression cassette that contains a second promoter, a TK-encoding sequence, a linker peptide-encoding sequence, and a second fluorescent protein-encoding sequence, where the expression of TK from the second expression cassette enables the H129-derived recombinant defective HSV-1 virus to replicate; wherein the first and second fluorescent protein-encoding sequences encode different fluorescent proteins.