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|1. (WO2018082085) MICROSCOPE IMAGE ACQUISITION METHOD BASED ON SEQUENCE SLICE|
|Applicants:||INSTITUTE OF AUTOMATION, CHINESE ACADEMY OF SCIENCES
|Title:||MICROSCOPE IMAGE ACQUISITION METHOD BASED ON SEQUENCE SLICE|
A microscope image acquisition method based on a sequence slice, comprising acquiring a sequence slice sample and a navigation map thereof (S100); using the method of image processing and machine learning to perform identification mark on the sequence slice sample in the navigation map (S110); placing the sequence slice sample in a microscope, navigating and locating any of the pixel points in the navigation map to the microscope field center (S120); locating the sequence slice sample in a low-resolution field, stapling sample acquisition parameters (S130); recording, on the basis of the stapling of the sample acquisition parameters, the relative position relationship between the center point of a high-resolution acquisition area and the center point after matching the sample template (S140); performing continuous image acquisition of the sample point in combination with the sample acquisition parameters and the relative position relationship (S150). The technical solution solves the technical problem of how to efficiently complete the automatic acquisition of a sample area image of interest, enabling the automated imaging of continuous areas of large-scale serial samples under the microscope with an automation control interface.