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1. (WO2018081757) RAPID POLYMERIZATION OF POLYPHENOLS

Pub. No.:    WO/2018/081757    International Application No.:    PCT/US2017/059131
Publication Date: Fri May 04 01:59:59 CEST 2018 International Filing Date: Tue Oct 31 00:59:59 CET 2017
IPC: C12Q 1/25
C12Q 1/28
G01N 33/48
G01N 33/50
G01N 33/53
G01N 33/531
G01N 33/532
G01N 33/535
G01N 33/536
G01N 33/577
Applicants: UNIVERSITY OF WASHINGTON
Inventors: GAO, Xiaohu
LI, Junwei
Title: RAPID POLYMERIZATION OF POLYPHENOLS
Abstract:
This disclosure provides a method for polymerizing polyphenols to provide polyphenol polymers using peroxidase and similar catalysis. In various aspects, it provides a method for polymerizing a polyphenol (e.g., polydopamine or a derivative or conjugate thereof) on a surface comprising polymerizing the polyphenol, a method for detecting an analyte comprising polymerizing a polyphenol, and an assay kit comprising a polyphenol (e.g., dopamine or a dopamine derivative). In one embodiment, a method for polymerizing a polyphenol includes contacting the polyphenol and an oxidant with an enzyme having peroxidase-like activity, under conditions sufficient to polymerize the polyphenol. In another embodiment, a method for depositing a polyphenol polymer (e.g., a polydopamine) includes providing, at a target site, an enzyme having peroxidase-like activity immobilized at the surface; and polymerizing, at the target site, a polyphenol in the presence of an oxidant and the enzyme to provide the polyphenol polymer, deposited on the surface.