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1. (WO2018081666) METHODS OF SINGLE DNA/RNA MOLECULE COUNTING
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Pub. No.: WO/2018/081666 International Application No.: PCT/US2017/058912
Publication Date: 03.05.2018 International Filing Date: 28.10.2017
IPC:
C12N 15/10 (2006.01) ,C12N 15/11 (2006.01) ,C12P 19/34 (2006.01) ,C12Q 1/06 (2006.01) ,C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
06
Quantitative determination
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants: SILGENTECH INC.[US/US]; 1315 Elsona Drive Sunnyvale, California 94087, US
Inventors: LIN, Meihong; US
Agent: SUN-HOFFMAN, Lin; US
Priority Data:
15/358,07621.11.2016US
62/414,62828.10.2016US
Title (EN) METHODS OF SINGLE DNA/RNA MOLECULE COUNTING
(FR) PROCÉDÉS DE COMPTAGE DE MOLÉCULES SIMPLES D'ADN/ARN
Abstract:
(EN) A method for counting single- or double-stranded polynucleotide molecules is provided. A plurality of double-stranded polynucleotide fragments is obtained from original single- or double-stranded polynucleotide molecules. The ends of the polynucleotide fragments can be modified to have overhangs suitable for ligation. Two single-stranded adaptors or one double-stranded polynucleotide linker are ligated to both ends of each of the double-stranded polynucleotide fragments to form single- or double-stranded circular polynucleotide molecules, which can be linearly amplified to form single-stranded nanoballs. The circular polynucleotide molecules before the amplification, or the single-stranded nanoballs obtained in the amplification, can be identified and quantified on a gene detection platform, thereby obtaining a counting of the original single- or double-stranded polynucleotide molecules.
(FR) L'invention concerne un procédé de comptage de molécules polynucléotidiques simple brin ou double brin. Une pluralité de fragments polynucléotidiques double brin est obtenue à partir de molécules initiales polynucléotidiques simple brin ou double brin. Les extrémités des fragments polynucléotidiques peuvent être modifiées pour présenter des parties qui dépassent, appropriées pour la ligature. Deux adaptateurs simple brin ou un lieur polynucléotidique double brin sont ligaturés aux deux extrémités de chacun des fragments polynucléotidiques double brin pour former des molécules polynucléotidiques circulaires simple ou double brin, qui peuvent être amplifiées de manière linéaire pour former des nanobilles simple brin. Les molécules polynucléotidiques circulaires avant l'amplification, ou les nanobilles simple brin obtenues dans l'amplification, peuvent être identifiées et quantifiées sur une plate-forme de détection de gène, ce qui permet d'obtenir un comptage des molécules initiales polynucléotidiques simple ou double brin.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)