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1. (WO2018081604) METHODS FOR SINGLE-STRANDED NUCLEIC ACID LIBRARY PREPARATION
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/081604 International Application No.: PCT/US2017/058821
Publication Date: 03.05.2018 International Filing Date: 27.10.2017
Chapter 2 Demand Filed: 28.08.2018
IPC:
C12Q 1/6806 (2018.01) ,C12N 15/10 (2006.01)
[IPC code unknown for C12Q 1/6806]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants: GRAIL, INC.[US/US]; 1525 O' Brien Drive Menlo Park, California 94025, US
Inventors: JAMSHIDI, Arash; --
AMINI, Hamed; --
Agent: PELLETIER, Benjamin C.; US
Priority Data:
62/414,52828.10.2016US
Title (EN) METHODS FOR SINGLE-STRANDED NUCLEIC ACID LIBRARY PREPARATION
(FR) PROCÉDÉS DE PRÉPARATION DE BIBLIOTHÈQUES D'ACIDES NUCLÉIQUES MONOCATÉNAIRES
Abstract:
(EN) Aspects of the invention relate to methods and compositions for preparing and analyzing a single-stranded sequencing library from a double-stranded DNA (e.g., double-stranded cfDNA) sample. In some embodiments, the sample includes double-stranded DNA (dsDNA) molecules, and damaged dsDNA (e.g., nicked dsDNA) molecules. In some embodiments, the sample includes single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information, including strand-pairing and connectivity information, from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared using conventional methods.
(FR) Des aspects de l'invention concernent des procédés et des compositions pour préparer et analyser une bibliothèque de séquençage monocaténaire à partir d'un échantillon d'ADN double brin (par exemple, un ADNcf double brin). Dans certains modes de réalisation, l'échantillon comprend des molécules d'ADN double brin (ADN db), et des molécules d'ADNdb endommagées (par exemple, des molécules d'ADNdb cassées). Dans certains modes de réalisation, l'échantillon comprend des molécules d'ADN simple brin (ADNsb). Les procédés selon l'invention facilitent la collecte d'informations, y compris des informations d'appariement de brins et de connectivité, à partir des molécules d'ADNdb, d'ADNsb et d'ADN endommagées (par exemple ADN cassé) présentes dans un échantillon, ce qui donne de meilleures informations relatives au diagnostic par rapport aux bibliothèques de séquençage qui sont préparées selon des procédés traditionnels.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)