Search International and National Patent Collections

1. (WO2018081039) MODULAR EXTRACELLULAR SENSOR ARCHITECTURE FOR REGULATING GENES

Pub. No.:    WO/2018/081039    International Application No.:    PCT/US2017/057968
Publication Date: Fri May 04 01:59:59 CEST 2018 International Filing Date: Wed Oct 25 01:59:59 CEST 2017
IPC: C12N 15/09
C12N 15/63
C12N 9/22
C12Q 1/6876
Applicants: NORTHWESTERN UNIVERSITY
Inventors: LEONARD, Joshua, N.
SCHWARZ, Kelly, A.
Title: MODULAR EXTRACELLULAR SENSOR ARCHITECTURE FOR REGULATING GENES
Abstract:
Disclosed are systems, components of systems, and methods for sensing extracellular ligands and/or modulating expression of an endogenous or exogenous gene in a cell. In some embodiments, the disclosed systems and methods comprise or utilize first and second exogenous extracellular sensors and/or nucleic acid sequences encoding the first and second exogenous extracellular sensors, wherein the first exogenous extracellular sensor comprises: a) a ligand binding domain; b) a transmembrane domain; c) a protease cleavage site; and d) a functional domain comprising an RNA-binding subdomain fused to a transcription regulatory subdomain; and the second exogenous extracellular sensor comprises: e) a ligand binding domain, f) a transmembrane domain, and g) a protease domain that cleaves the protease cleavage site of the first exogenous extracellular receptor.