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1. (WO2018080148) THREE-DIMENSIONAL CELL CULTURE SYSTEM COMPRISING PEG-BASED HYDROGEL
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Pub. No.: WO/2018/080148 International Application No.: PCT/KR2017/011802
Publication Date: 03.05.2018 International Filing Date: 25.10.2017
IPC:
C12N 5/00 (2006.01) ,C12N 5/071 (2010.01) ,G01N 33/50 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Applicants:
주식회사 세바바이오텍 SHEBAHBIOTECH INC. [KR/KR]; 강원도 춘천시 소양강로 40, 1동 405호, 406호, 408호, 416호 405ho, 406ho, 408gh, 416ho, 1dong, 40, Soyanggang-ro, Chuncheon-si, Gangwon-do 06645, KR
Inventors:
이승태 LEE, Seung Tae; KR
임혜원 LIM, Hye Won; KR
윤정임 YUN, Jung Im; KR
김민성 KIM, Min Seong; KR
Agent:
김영호 KIM, Young Ho; KR
Priority Data:
10-2016-014348631.10.2016KR
Title (EN) THREE-DIMENSIONAL CELL CULTURE SYSTEM COMPRISING PEG-BASED HYDROGEL
(FR) SYSTÈME DE CULTURE CELLULAIRE TRIDIMENSIONNEL COMPRENANT UN HYDROGEL À BASE DE PEG
(KO) PEG-기반 하이드로젤을 포함하는 3차원 세포배양 시스템
Abstract:
(EN) The present invention relates to a three-dimensional cell culture system comprising a polyethylene glycol (PEG)-based hydrogel, wherein the PEG-based hydrogel is prepared by mixing an adhesion peptide and a crosslinker to polyethylene glycol-vinylsulfone (PEG-VS) that is a PEG to which vinylsulfone is bonded. The system can cultivate dermal fibroblasts, melanocytes or keratinocytes, which are skin cells, and thus can be effectively used to measure the activity of skin cells.
(FR) La présente invention concerne un système de culture cellulaire tridimensionnel comprenant un hydrogel à base de polyéthylène glycol (PEG), où l'hydrogel à base de PEG est préparé par mélange d'un peptide d'adhérence et d'un agent de réticulation à une polyéthylène glycol-vinylsulfone (PEG-VS) qui est un PEG auquel une vinylsulfone est liée. Le système permet de cultiver des fibroblastes dermiques, des mélanocytes ou des kératinocytes, qui sont des cellules cutanées, et peut par conséquent être efficacement utilisé pour mesurer l'activité des cellules cutanées.
(KO) 본 발명은 PEG(polyethylene glycol)-기반 하이드로젤을 포함하는 3차원 세포배양 시스템에 관한 것으로서, 상기 PEG-기반 하이드로젤은 비닐술폰(vinylsulfone)이 결합된 PEG(polyethylene glycol)인 PEG-VS(polyethylene glycol-vinylsulfone)에 부착 펩타이드(adhesion peptide) 및 가교제(crosslinker)를 혼합하여 제조되며, 상기 시스템은 피부세포인 진피섬유아세포, 멜라노세포 또는 케라틴세포를 배양할 수 있어 피부세포의 활성을 측정하는데 유용하게 사용될 수 있다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)