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1. (WO2018067826) GENERATING MATURE LINEAGES FROM INDUCED PLURIPOTENT STEM CELLS WITH MECP2 DISRUPTION

Pub. No.:    WO/2018/067826    International Application No.:    PCT/US2017/055353
Publication Date: Fri Apr 13 01:59:59 CEST 2018 International Filing Date: Fri Oct 06 01:59:59 CEST 2017
IPC: C12N 5/0783
C12N 5/0784
C12N 5/0789
C12N 15/85
Applicants: FUJIFILM CELLULAR DYNAMICS, INC.
Inventors: RAJESH, Deepika
STROUSE, Anne
BURTON, Sarah
MUNN, Christie
SWANSON, Bradley
Title: GENERATING MATURE LINEAGES FROM INDUCED PLURIPOTENT STEM CELLS WITH MECP2 DISRUPTION
Abstract:
Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.