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Pub. No.:    WO/2018/066910    International Application No.:    PCT/KR2017/010907
Publication Date: 12.04.2018 International Filing Date: 29.09.2017
C12Q 1/68 (2006.01)
Applicants: GENOMICTREE, INC. [KR/KR]; 44-6, Techno 10-ro Yuseong-gu Daejeon 34027 (KR)
Inventors: AN, Sungwhan; (KR).
OH, TaeJeong; (KR)
Agent: LEE, Cheo Young; (KR).
CHANG, Je Hwan; (KR)
Priority Data:
10-2016-0129110 06.10.2016 KR
(KO) 메틸화 DNA 다중 검출방법
Abstract: front page image
(EN)The present invention relates to a method of multiple detection of methylation in target DNA and a composition for detecting the methylation of target DNA and, more particularly, to a method for detecting the methylation of target DNA, by constructing an oligonucleotide including a target-specific sequence capable of binding complementarily to the target DNA and a universal primer incapable of binding complementarily to the target DNA, linearly amplifying the target DNA with the oligonucleotide serving as a primer, and amplifying the linearly amplified target DNA by means of the oligonucleotide capable of binding complementarily to the linearly amplified DNA, the universal primer, and a probe.
(FR)La présente invention concerne un procédé de détection multiple de la méthylation dans un ADN cible et une composition permettant de détecter la méthylation d'un ADN cible et, plus particulièrement, un procédé de détection de la méthylation d'un ADN cible, par construction d'un oligonucléotide comprenant une séquence spécifique d'une cible apte à se lier de manière complémentaire à l'ADN cible et une amorce universelle incapable de se lier de manière complémentaire à l'ADN cible, amplification linéaire de l'ADN cible avec l'oligonucléotide servant d'amorce, et amplification de l'ADN cible amplifié linéairement au moyen de l'oligonucléotide apte à se lier de manière complémentaire à l'ADN amplifié linéairement, à l'amorce universelle et à une sonde.
(KO)본 발명은 타겟 DNA의 메틸화를 다중으로 검출하는 방법 및 타겟 DNA의 메틸화 검출을 위한 조성물에 관한 것으로, 더욱 상세하게는 타겟 DNA와 상보적으로 결합할 수 있는 타겟 특이적 서열 및 타겟 DNA에 상보적으로 결합하지 않는 유니버셜 프라이머를 포함하는 올리고뉴클레오타이드를 제작하여, 이를 프라이머로 사용함으로써 타겟 DNA를 선형 증폭하고, 선형 증폭된 타겟 DNA를 선형 증폭된 DNA에 상보적으로 결합할 수 있는 올리고뉴클레오타이드, 유니버셜 프라이머 및 프로브를 이용하여 증폭함으로써, 타겟 DNA의 메틸화를 검출하는 방법에 관한 것이다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)