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1. (WO2018065299) NUCLEIC ACID SEQUENCING USING NANOTRANSISTORS

Pub. No.:    WO/2018/065299    International Application No.:    PCT/EP2017/074682
Publication Date: Fri Apr 13 01:59:59 CEST 2018 International Filing Date: Fri Sep 29 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: F. HOFFMANN-LA ROCHE AG
ROCHE DIAGNOSTICS GMBH
ROCHE SEQUENCING SOLUTIONS, INC.
Inventors: ASTIER, Yann
Title: NUCLEIC ACID SEQUENCING USING NANOTRANSISTORS
Abstract:
Embodiments may include a method of determining a nucleic acid sequence. The nucleic acid may be DNA. The method may include forming, by a polymerase, a double-stranded nucleic acid molecule from a first nucleic acid strand and a second nucleic acid strand. Forming the double-stranded nucleic acid molecule may include adding a first compound to the second nucleic acid strand. The first compound may include a nucleotide attached to a third nucleic acid strand. The third nucleic acid strand may be attached to a moiety. The method may also include detaching the nucleotide from the third nucleic acid strand and the moiety. The method may further include measuring a change in an electrical characteristic of a transistor resulting from the moiety. Additionally, the method may include identifying the nucleotide based on the measured change in the electrical characteristic.