WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Options
Query Language
Stem
Sort by:
List Length
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/064571 International Application No.: PCT/US2017/054494
Publication Date: 05.04.2018 International Filing Date: 29.09.2017
IPC:
C12Q 1/48 (2006.01) ,C12P 21/04 (2006.01) ,G01N 33/53 (2006.01) ,C12N 15/00 (2006.01) ,G01N 33/566 (2006.01) ,G01N 33/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
48
involving transferase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
04
Cyclic or bridged peptides or polypeptides, e.g. bacitracin
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
566
using specific carrier or receptor proteins as ligand binding reagent
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
Applicants: UNIVERSITY OF MIAMI[US/US]; 1951 NW 7th Avenue Miami, FL 33136, US
Inventors: ZHANG, Fangliang; US
KUMAR, Akhilesh; US
BIRNBAUM, Michael, D.; US
Agent: KISSLING, Heather, R.; US
Priority Data:
62/402,60530.09.2016US
Title (EN) METHODS TO DETECT DNA MUTAGENESIS
(FR) PROCÉDÉS DE DÉTECTION DE MUTAGÉNÈSE D'ADN
Abstract:
(EN) The disclosure provides a method comprising introducing into a cell an expression vector comprising a nucleic acid sequence encoding a reporter protein fused to an arginyltransferase 1 substrate; measuring arginylation and reporter protein levels; and calculating the ratio of arginylated substrate to reporter protein; and a method comprising introducing into a cell a reporter plasmid comprising a nucleic acid encoding a promoter, a first reporter protein, an internal ribosome entry site (IRES), and a second reporter protein, wherein the nucleic acid encoding the first reporter protein comprises a premature stop codon and mutation of the stop codon results in first reporter protein expression, or the nucleic acid encoding the second reporter protein comprises a premature stop codon and mutation of the stop codon results second reporter protein expression; measuring the first reporter protein and the second reporter protein; and calculating the ratio of first reporter protein to second reporter protein.
(FR) La présente invention concerne un procédé comprenant l'introduction dans une cellule d'un vecteur d'expression comprenant une séquence d'acide nucléique codant pour une protéine rapporteur fusionnée à un substrat d'arginyltransférase 1; la mesure des taux d'arginylation et de la protéine rapporteur; et le calcul du rapport entre le substrat arginylé et la protéine rapporteur. L'invention concerne également un procédé comprenant l'introduction dans une cellule d'un plasmide rapporteur comprenant un acide nucléique codant pour un promoteur, une première protéine rapporteur, un site d'entrée interne des ribosomes (IRES), et une seconde protéine rapporteur, l'acide nucléique codant pour la première protéine rapporteur comprenant un codon d'arrêt prématuré et la mutation du codon d'arrêt conduisant à l'expression de la première protéine rapporteur, ou l'acide nucléique codant pour la seconde protéine rapporteur comprenant un codon d'arrêt prématuré et la mutation du codon d'arrêt conduisant à l'expression de la seconde protéine rapporteur; la mesure de la première protéine rapporteur et de la seconde protéine rapporteur; et le calcul du rapport entre la première protéine rapporteur et la seconde protéine rapporteur.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)