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1. (WO2018061877) NUCLEIC ACID EXTRACTION METHOD AND KIT USING SAME

Pub. No.:    WO/2018/061877    International Application No.:    PCT/JP2017/033741
Publication Date: Fri Apr 06 01:59:59 CEST 2018 International Filing Date: Wed Sep 20 01:59:59 CEST 2017
IPC: C12Q 1/68
C12N 15/09
Applicants: EIKEN KAGAKU KABUSHIKI KAISHA
栄研化学株式会社
Inventors: SATO Masaki
佐藤 正樹
YONEKAWA Toshihiro
米川 俊広
Title: NUCLEIC ACID EXTRACTION METHOD AND KIT USING SAME
Abstract:
Disclosed is a method for extracting a nucleic acid from a biological sample. This method is provided with: (i) a step for mixing a biological sample, an anionic surfactant, and a chaotropic compound to obtain a solution; (ii) a step for mixing the solution, an alkanol having a boiling point exceeding 75°C, a chaotropic compound, and silica particles to cause a nucleic acid to be adsorbed on thee silica particles; (iii) a step for rinsing the silica particles on which the nucleic acid is adsorbed using a rinsing liquid; and (iv) a step for eluting the nucleic acid adsorbed on the silica particles using an eluting liquid; at least steps (i) and (ii) being performed at a temperature exceeding 75°C.