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1. (WO2018060589) METHOD FOR COUNTING WHITE BLOOD CELLS IN A SAMPLE

Pub. No.:    WO/2018/060589    International Application No.:    PCT/FR2017/052591
Publication Date: Fri Apr 06 01:59:59 CEST 2018 International Filing Date: Wed Sep 27 01:59:59 CEST 2017
IPC: G06K 9/00
G01N 15/14
G03H 1/04
G03H 1/08
G01N 15/10
G01N 15/00
Applicants: COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES
HORIBA ABX SAS
UNIVERSITÉ D'AIX-MARSEILLE
Inventors: ALLIER, Cédric
HERVE, Lionel
MOREL, Sophie
ISEBE, Damien
DRANCOURT (PR.), Michel
ALI CHERIF, Anais
Title: METHOD FOR COUNTING WHITE BLOOD CELLS IN A SAMPLE
Abstract:
The invention relates to a method for determining the number of white blood cells in a sample. The method is based on an image, or hologram, of the sample, obtained by an image sensor when the sample is illuminated by a light source. The hologram is reconstructed holographically, such as to obtain a complex image, known as the reference complex image, representing a light wave transmitted by the sample, in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, such as to produce a plurality of so-called secondary complex images, forming a stack of images, from which a profile is determined describing the evolution of an optical characteristic of the light wave transmitted by the sample along the axis of propagation of said light wave. The presence of white blood cells and the number thereof are determined according to the aforementioned profiles.