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|1. (WO2018060589) METHOD FOR COUNTING WHITE BLOOD CELLS IN A SAMPLE|
|Applicants:||COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES
HORIBA ABX SAS
DRANCOURT (PR.), Michel
ALI CHERIF, Anais
|Title:||METHOD FOR COUNTING WHITE BLOOD CELLS IN A SAMPLE|
The invention relates to a method for determining the number of white blood cells in a sample. The method is based on an image, or hologram, of the sample, obtained by an image sensor when the sample is illuminated by a light source. The hologram is reconstructed holographically, such as to obtain a complex image, known as the reference complex image, representing a light wave transmitted by the sample, in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, such as to produce a plurality of so-called secondary complex images, forming a stack of images, from which a profile is determined describing the evolution of an optical characteristic of the light wave transmitted by the sample along the axis of propagation of said light wave. The presence of white blood cells and the number thereof are determined according to the aforementioned profiles.