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1. (WO2018060415) FULL LENGTH KINASE ACTIVITY-CONFORMATION REPORTER

Pub. No.:    WO/2018/060415    International Application No.:    PCT/EP2017/074761
Publication Date: Fri Apr 06 01:59:59 CEST 2018 International Filing Date: Sat Sep 30 01:59:59 CEST 2017
IPC: G01N 33/68
Applicants: UNIVERSITÄT INNSBRUCK
Inventors: STEFAN, Eduard
MAYERHOFER, Johanna
Title: FULL LENGTH KINASE ACTIVITY-CONFORMATION REPORTER
Abstract:
The present invention provides a reporter for a protein fragment complementation assay characterized in that the reporter is a fused protein comprising a first fragment, a second fragment and a protein kinase sequence section, wherein the first fragment and the second fragment are derived from different sections of the same split protein, and wherein the protein kinase sequence section intervenes between the first fragment and the second fragment and wherein the kinase sequence section comprises a kinase domain sequence and one or more regulatory sequence(s). Further the invention provides polynucleotides and cells encoding for the reporter as well as methods of conducting a protein fragment complementation assay with the reporter according to the invention.