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1. (WO2018060249) METHOD FOR LABELING OLIGONUCLEOTIDE PROBES
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Pub. No.: WO/2018/060249 International Application No.: PCT/EP2017/074505
Publication Date: 05.04.2018 International Filing Date: 27.09.2017
IPC:
C12Q 1/68
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS [DE/DE]; Im Neuenheimer Feld 280 69120 Heidelberg, DE
Inventors:
CHENG, Yong-Sheng; DE
LIU, Hai-Kun; DE
Agent:
KUTTENKEULER, David; BOEHMERT & BOEHMERT Pettenkoferstrasse 22 80336 Munich, DE
Priority Data:
16190862.927.09.2016EP
Title (EN) METHOD FOR LABELING OLIGONUCLEOTIDE PROBES
(FR) PROCÉDÉ DE MARQUAGE DE SONDES OLIGONUCLÉOTIDIQUES
Abstract:
(EN) The present invention provides a novel method for labelling nucleic acid probes. The method uses a ligase catalysed reaction to connect a nucleic acid probe with pre-prepared nucleic acid label carrier molecules under the presence of a stabilizing complementary splint oligonucleotide. The method allows for an easy, cheap and fast labelling of multiple probes with multiple different labels. In this way, the costs and effort for the generation of single molecule Fluorescent In Situ Hybridization (smFISH) assays was significantly reduced. The invention further provides methods for the generation of FISH libraries and labelling kits comprising the novel tools of the invention.
(FR) La présente invention concerne un nouveau procédé de marquage de sondes d'acide nucléique. Le procédé utilise une réaction catalysée par une ligase pour relier une sonde d'acide nucléique à des molécules de support de marqueur d'acide nucléique pré-préparées en présence d'un oligonucléotide attelle complémentaire de stabilisation. Le procédé permet un marquage facile, peu coûteux et rapide de multiples sondes avec de multiples marqueurs différents. De cette manière, les coûts et l'effort pour la génération d'essais d'hybridation in situ en fluorescence en molécule unique (smFISH) ont été significativement réduits. L'invention concerne en outre des procédés de génération de banques FISH et des kits de marquage comprenant les nouveaux outils de l'invention.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)