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1. (WO2018059651) METHOD FOR MODIFYING THE RESISTANCE PROFILE OF SPINACIA OLERACEA TO DOWNY MILDEW

Pub. No.:    WO/2018/059651    International Application No.:    PCT/EP2016/001621
Publication Date: Fri Apr 06 01:59:59 CEST 2018 International Filing Date: Sat Oct 01 01:59:59 CEST 2016
IPC: A01H 5/12
C12N 15/82
C12Q 1/68
Applicants: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.
Inventors: KOCK, Vincent Laurens Adrianus
FEITSMA, Johannes Geert Jan
FRIJTERS, Raoul Jacobus Johannes
Title: METHOD FOR MODIFYING THE RESISTANCE PROFILE OF SPINACIA OLERACEA TO DOWNY MILDEW
Abstract:
The present invention relates to a method for modifying the resistance profile of a spinach plant to Peronospora farinosa f. sp. spinaciae, comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS- LRR protein that comprises in its amino acid sequence: the motif "MAEIGYSVC" at its N- terminus, and the motif "KWMCLR" for alpha-type WOLF proteins or "HVGCVVDR" for beta- type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance to Peronospora farinosa f. sp. spinaciae in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races of Peronospora farinosa f. sp. spinaciae in a spinach plant and to primers for use in these methods.