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1. (WO2018058249) SYSTEM FOR IDENTIFYING AND TARGETING INDIVIDUAL CELLS WITHIN A HETEROGENEOUS POPULATION FOR SELECTIVE EXTRACTION OF CELLULAR CONTENT
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Pub. No.: WO/2018/058249 International Application No.: PCT/CA2017/051158
Publication Date: 05.04.2018 International Filing Date: 29.09.2017
IPC:
C12M 1/34 (2006.01) ,B81B 1/00 (2006.01) ,C12M 1/33 (2006.01) ,C12M 1/42 (2006.01) ,C12N 1/06 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/00 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 1/28 (2006.01) ,G01N 1/40 (2006.01) ,G01N 35/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
B PERFORMING OPERATIONS; TRANSPORTING
81
MICRO-STRUCTURAL TECHNOLOGY
B
MICRO-STRUCTURAL DEVICES OR SYSTEMS, e.g. MICRO-MECHANICAL DEVICES
1
Devices without movable or flexible elements, e.g. micro-capillary devices
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
33
Disintegrators
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
42
Apparatus for the treatment of micro-organisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
06
Lysis of micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
40
Concentrating samples
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
Applicants:
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO [CA/CA]; 100 College Street Toronto, Ontario M5G 1L5, CA
SINAI HEALTH SYSTEM [CA/CA]; 600 University Avenue Toronto, Ontario M5G 1X5, CA
Inventors:
WHEELER, Aaron Ray; CA
CHAMBERLAIN, Michael Dean; CA
LAMANNA, Julian Lucas; CA
DRYDEN, Michael David Murdoch; CA
KOLOMIETZ, Elena; CA
CHITAYAT, David; CA
Agent:
HILL & SCHUMACHER; 264 Avenue Road Toronto, Ontario M4V 2G7, CA
Priority Data:
62/402,20830.09.2016US
Title (EN) SYSTEM FOR IDENTIFYING AND TARGETING INDIVIDUAL CELLS WITHIN A HETEROGENEOUS POPULATION FOR SELECTIVE EXTRACTION OF CELLULAR CONTENT
(FR) SYSTÈME D'IDENTIFICATION ET DE CIBLAGE DE CELLULES INDIVIDUELLES DANS UNE POPULATION HÉTÉROGÈNE POUR L'EXTRACTION SÉLECTIVE DE CONTENU CELLULAIRE
Abstract:
(EN) The present disclosure provides a system and method for identifying and targeting individual cells within a cell population for selective extraction of cellular content and a digital microfluidic device having at least one hydrophilic site for receiving cells, an imaging system including a stage for receiving the digital microfluidic device and an imaging module for identifying at least one targeted cell among the cells at the at least one hydrophilic site. The system includes a pulsed laser source for laser lysing the targeted cell thereby releasing the cell content to produce a lysate. A control system controls the pulsed laser source, the imaging system and the digital microfluidic device and is programmed for coordinating steps of i) movement of droplets on the digital microfluidic device, ii) selection of the at least one targeted cell to be lysed located at the at least one hydrophilic site, iii) illumination of the at least one selected targeted cell by the pulsed laser source to lyse the at least one selected targeted cell to produce lysate, and iv) collection of the lysate.
(FR) La présente invention concerne un système et un procédé d'identification et de ciblage de cellules individuelles dans une population de cellules pour l'extraction sélective de contenu cellulaire et un dispositif microfluidique numérique comportant au moins un site hydrophile pour recevoir des cellules, un système d'imagerie comprenant un étage pour recevoir le dispositif microfluidique numérique et un module d'imagerie pour identifier au moins une cellule ciblée parmi les cellules au niveau du ou des sites hydrophiles. Le système comprend une source laser pulsée destinée à lyser par laser la cellule ciblée, ce qui libère le contenu cellulaire pour produire un lysat. Un système de commande régule la source laser pulsée, le système d'imagerie et le dispositif microfluidique numérique, et est programmé pour coordonner les étapes i) de mouvement de gouttelettes sur le dispositif microfluidique numérique, ii) de sélection de la ou des cellules ciblées à lyser situées au niveau du ou des sites hydrophiles, iii) d'éclairage de la ou des cellules ciblées sélectionnées par la source laser pulsée pour lyser la ou les cellules ciblées sélectionnées pour produire un lysat, et iv) de recueil du lysat.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)