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1. (WO2018058023) METHODS FOR IDENTIFYING PROTEINS THAT BIND LIGANDS

Pub. No.:    WO/2018/058023    International Application No.:    PCT/US2017/053207
Publication Date: Fri Mar 30 01:59:59 CEST 2018 International Filing Date: Tue Sep 26 01:59:59 CEST 2017
IPC: G01N 33/68
G01N 33/94
G01N 33/53
Applicants: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
BONDARENKO, Andrey
Inventors: BONDARENKO, Andrey
YATES, Nathan A.
MULLETT, Steven James
BELL-TEMIN, Harris B.
Title: METHODS FOR IDENTIFYING PROTEINS THAT BIND LIGANDS
Abstract:
Provided herein are methods of identifying a protein capable of binding a ligand, the method comprising: (a) contacting the ligand with two or more samples comprising a plurality of proteins in a solution; (b) separating the proteins bound to the ligand ("bound proteins") from the proteins that are not bound to the ligand ("unbound proteins") in each sample; (c) denaturing and digesting the bound proteins to form a plurality of peptides in each sample; (d) quantifying a plurality of molecular features contained in the plurality of peptides in each sample, wherein the molecular features are defined as having a mass to charge ratio, retention time, and peak intensity as measured by mass spectrometry; and (e) ranking the molecular features that exhibit a statistically significant difference in quantity between the samples contacted with the ligand and a sample that is not contacted with the ligand ("statistically significant molecular feature").