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1. (WO2018054783) METHOD FOR QUANTIFYING AND/OR DETECTING HUMAN MALE DNA
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2018/054783    International Application No.:    PCT/EP2017/073311
Publication Date: 29.03.2018 International Filing Date: 15.09.2017
IPC:
C12Q 1/68 (2006.01)
Applicants: QIAGEN GMBH [DE/DE]; Qiagen Str. 1 40724 Hilden (DE)
Inventors: VRANES, Miroslav; (DE).
PEIST, Ralf; (DE).
SCHERER, Mario; (DE).
CORNELIUS, Stefan Otto; (DE).
KÖNIG, Margaretha; (DE)
Agent: CH KILGER ANWALTSPARTNERSCHAFT MBB; Fasanenstr. 29 10719 Berlin (DE)
Priority Data:
16189210.4 22.09.2016 EP
Title (EN) METHOD FOR QUANTIFYING AND/OR DETECTING HUMAN MALE DNA
(FR) PROCÉDÉ DE QUANTIFICATION ET/OU DE DÉTECTION D'ADN MÂLE HUMAIN
Abstract: front page image
(EN)According to a first aspect of the present invention, a method is provided for detecting and/or quantifying male genomic DNA in a sample, wherein the method comprises the step of amplification of a multicopy locus within the human Y-chromosome (MCL-Y), wherein said locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs (bp). A second aspect of the present invention relates to a primer or primer pair which hybridizes under stringent conditions to a sequence according to SEQ ID NO. 3 and/or any of 4 to 11. The invention also relates to a kit.
(FR)Selon un premier aspect, la présente invention concerne un procédé de détection et/ou de quantification d'ADN génomique mâle dans un échantillon, le procédé comprenant l'étape d'amplification d'un locus multicopie au sein du chromosome Y humain (MCL-Y), ledit locus partageant au moins 80 % d'identité de séquence avec une séquence selon SEQ ID NO 3 sur un portion d'au moins 60 paires de bases (pb). Un deuxième aspect de la présente invention concerne une amorce ou une paire d'amorces qui s'hybride dans des conditions stringentes à une séquence selon SEQ ID NO 3 et/ou l'une quelconque séquence 4 à 11. L'invention concerne également un kit.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)