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1. (WO2018053463) ARTIFICIAL ANTIGEN PRESENTING CELLS FOR GENETIC ENGINEERING OF IMMUNE CELLS
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Pub. No.: WO/2018/053463 International Application No.: PCT/US2017/052152
Publication Date: 22.03.2018 International Filing Date: 19.09.2017
IPC:
C07K 14/725 (2006.01) ,C07K 19/00 (2006.01) ,C12N 15/09 (2006.01) ,A61K 35/17 (2015.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
725
T-cell receptors
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
19
Hybrid peptides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
[IPC code unknown for A61K 35/17]
Applicants:
H. LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE, INC. [US/US]; 12902 Magnolia Drive Tampa, FL 33612-9497, US
Inventors:
DAVILA, Marco; US
Agent:
CLEVELAND, Janell, T.; US
HALL, Miles, E.; US
Priority Data:
62/396,55519.09.2016US
62/400,96128.09.2016US
Title (EN) ARTIFICIAL ANTIGEN PRESENTING CELLS FOR GENETIC ENGINEERING OF IMMUNE CELLS
(FR) CELLULES PRÉSENTATRICES D'ANTIGÈNE ARTIFICIEL POUR L'INGÉNIERIE GÉNÉTIQUE DE CELLULES IMMUNITAIRES
Abstract:
(EN) Disclosed herein are methods of producing chimeric antigen receptor (CAR) T cells using substrates, such as artificial antigen presenting cells, containing on a surface a a heparin binding domain (HBD), anti-CD3 single chain antibodies, anti-CD28 single chain antibodies (scFv), and optionally anti-41BBL antibodies. Anti-CD3 and Anti-CD28 scFvs bind and activate expanding T cells ex vivo, while the Heparin Binding Domain binds the viral vector, thereby bringing the T cells into close proximity with virus for effective gene transfer. This is a less costly, renewable, modifiable, and efficacious alternative to coated beads and RetroNectin® for gene transfer.
(FR) L'invention concerne des procédés de production de lymphocytes T du récepteur d'antigène chimérique (CAR) à l'aide de substrats, tels que des cellules présentatrices d'antigène artificiel, contenant sur une surface un domaine de liaison à l'héparine (HBD), des anticorps à chaîne unique anti-CD3, des anticorps à chaîne unique anti-CD28 (scFv), et éventuellement des anticorps anti-41BBL. Les anticorps Anti-CD3 et Anti-CD28 scFvs se lient et activent les lymphocytes T à expansion ex vivo, tandis que le domaine de liaison à l'héparine se lie au vecteur viral, amenant ainsi les lymphocytes T à se rapprocher du virus pour un transfert de gène efficace. Il s'agit d'une alternative moins coûteuse, renouvelable, modifiable et efficace à des billes enrobées et à la RetroNectin® pour le transfert de gènes.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)