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1. (WO2018053308) UNIVERSAL METHOD FOR EXTRACTING NUCLEIC ACID MOLECULES FROM A DIVERSE POPULATION OF ONE OR MORE TYPES OF MICROBES IN A SAMPLE
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Pub. No.: WO/2018/053308 International Application No.: PCT/US2017/051849
Publication Date: 22.03.2018 International Filing Date: 15.09.2017
IPC:
C12N 15/10 (2006.01) ,B01J 20/24 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
J
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
20
Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
22
comprising organic material
24
Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
Applicants: SUN GENOMICS LLC[US/US]; 10210 Campus Point Drive, Suite 150 San Diego, CA 92121, US
Inventors: JAIN, Suneer; US
Agent: HAILE, Lisa, A.; US
HIERHOLZER, Matthew, J.; US
HWANG, Pamela, E.; US
JACKSON, Blake; US
LIMBACH, Alan, A.; US
Priority Data:
62/395,31615.09.2016US
62/412,78725.10.2016US
Title (EN) UNIVERSAL METHOD FOR EXTRACTING NUCLEIC ACID MOLECULES FROM A DIVERSE POPULATION OF ONE OR MORE TYPES OF MICROBES IN A SAMPLE
(FR) PROCÉDÉ UNIVERSEL D’EXTRACTION DE MOLÉCULES D’ACIDES NUCLÉIQUES À PARTIR D’UNE POPULATION DIVERSE D’UN OU PLUSIEURS TYPES DE MICROBES DANS UN ÉCHANTILLON
Abstract:
(EN) Disclosed herein are methods of extracting genetic material from a diverse population of one or more types of microbes in a sample. Microbes can be prokaryotes or eukaryotes and may include bacteria, archaea, fungi, protozoa, helminths, parasites, viruses, phages, and others. Extraction may be from a single sample and subsequent identification may be through a molecular method such as qPCR, PCR, RFLP, SSCP, allele specific PCR, targeted sequencing, pull down sequencing, whole shotgun sequencing, or other methods.
(FR) La présente invention concerne des procédés d’extraction de matériel génétique à partir d’une population diverse d’un ou plusieurs types de microbes dans un échantillon. Les microbes peuvent être des procaryotes ou des eucaryotes et peuvent comprendre des bactéries, des archées, des champignons, des protozoaires, des helminthes, des parasites, des virus, des phages, et d’autres. L’extraction peut être effectuée à partir d’un échantillon unique et l’identification ultérieure peut être effectuée à travers un procédé moléculaire tel que la qPCR, la PCR, le RFLP, le SSCP, la PCR spécifique à un allèle, le séquençage ciblé, le séquençage pull down, le séquençage à l'aveugle du génome entier, ou d’autres procédés.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)