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1. (WO2018053020) METHODS OF NON-DESTRUCTIVE NANOSTRAW INTRACELLULAR SAMPLING FOR LONGITUDINAL CELL MONITORING

Pub. No.:    WO/2018/053020    International Application No.:    PCT/US2017/051392
Publication Date: Fri Mar 23 00:59:59 CET 2018 International Filing Date: Thu Sep 14 01:59:59 CEST 2017
IPC: B82Y 40/00
C12M 1/00
C12M 1/34
C12M 3/00
C12Q 1/02
C12Q 1/68
G01N 27/00
Applicants: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Inventors: MELOSH, Nicholas, A.
CAO, Yuhong
HJORT, Karl, Martin
JONSSON, Amanda
Title: METHODS OF NON-DESTRUCTIVE NANOSTRAW INTRACELLULAR SAMPLING FOR LONGITUDINAL CELL MONITORING
Abstract:
Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.