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1. (WO2018052137) FINE PARTICLE DISPENSING DEVICE, FINE PARTICLE ANALYSIS DEVICE, REACTION DETECTION DEVICE, AND METHOD USING SAID DEVICES
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Pub. No.: WO/2018/052137 International Application No.: PCT/JP2017/033551
Publication Date: 22.03.2018 International Filing Date: 15.09.2017
IPC:
G01N 15/14 (2006.01) ,C12M 1/00 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 35/10 (2006.01) ,G01N 37/00 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
14
Electro-optical investigation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35
Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
10
Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
37
Details not covered by any other group of this subclass
Applicants: ON-CHIP BIOTECHNOLOGIES CO., LTD.[JP/JP]; 204, Nokodai TamaKoganei Venture Port, 2-24-16, Naka-cho, Koganei-shi, Tokyo 1840012, JP
Inventors: TAKEDA Kazuo; JP
FUJIMURA Yuu; JP
ISHIGE Masayuki; JP
INO Takahide; JP
BANSHO Yohsuke; JP
AKAGI Jin; JP
OSAWA Kosuke; JP
Agent: YAMAGUCHI Kenjiro; JP
MORITA Kenichi; JP
Priority Data:
2016-18144716.09.2016JP
Title (EN) FINE PARTICLE DISPENSING DEVICE, FINE PARTICLE ANALYSIS DEVICE, REACTION DETECTION DEVICE, AND METHOD USING SAID DEVICES
(FR) DISPOSITIF DE DISTRIBUTION DE PARTICULES FINES, DISPOSITIF D'ANALYSE DE PARTICULES FINES, DISPOSITIF DE DÉTECTION DE RÉACTION ET PROCÉDÉ UTILISANT LESDITS DISPOSITIFS
(JA) 微粒子分注装置、微粒子解析装置、及び反応検出装置、並びにそれらを用いる方法
Abstract:
(EN) The purpose of the present invention is to provide a technology for dispensing cells as single cells. Said purpose is achieved by a fine particle dispensing device that has a transparent hollow pipette for dispensing a sample liquid containing fine particles of the present invention, and has an image capturing means. The fine particle dispensing device comprises a means for capturing an image of a dispensing liquid inside a hollow pipette at least two times, a means for comparing the at least two captured images, differentiating between particulate-like matter that does not move and particulate-like matter that has moved among the particulate-like matter for which images were captured so as to discover floating particles, and a means for dispensing the sample liquid that contains a target number of floating particles.
(FR) La présente invention a pour objet de proposer une technologie pour la distribution de cellules sous la forme de cellules uniques. Ce but est atteint grâce à un dispositif de distribution de particules fines qui comporte une pipette creuse transparente pour la distribution d'un échantillon liquide contenant de fines particules de la présente invention, le dispositif comportant également un moyen de capture d'image. Le dispositif de distribution de particules fines comprend un moyen pour la capture, au moins à deux reprises, d'une image d'un liquide à distribuer se trouvant à l'intérieur d'une pipette creuse, un moyen de comparaison des au moins deux images capturées, permettant la différenciation entre une matière de type particulaire qui ne bouge pas et une matière de type particulaire qui a bougé au sein de la matière de type particulaire pour laquelle des images ont été capturées de façon à découvrir des particules flottantes, et un moyen de distribution de l'échantillon liquide qui contient un nombre cible de particules flottantes.
(JA) 本発明の目的は、細胞を単一細胞として分注する技術を提供することである。 前記課題は、本発明の微粒子を含む試料液を分注するための透明な中空ピペット、及び画像撮影手段を有する微粒子分注装置であって、中空ピペット中の分注液を2回以上撮影する手段、2つ以上の撮影した画像を比較し、撮影された微粒子様物のうち移動した微粒子様物を、移動しない微粒子様物と区別して浮遊粒子と判定する手段、及び目的の個数の浮遊粒子を含む試料液を分注する手段を有する、微粒子分注装置、及びによって解決することができる。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)