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1. (WO2018047884) METHOD FOR PREPARING MONONUCLEAR CELLS

Pub. No.:    WO/2018/047884    International Application No.:    PCT/JP2017/032186
Publication Date: Fri Mar 16 00:59:59 CET 2018 International Filing Date: Fri Sep 08 01:59:59 CEST 2017
IPC: C12N 5/0786
A61K 35/15
A61P 43/00
Applicants: YONEMITSU Yoshikazu
米満 吉和
GAIA BIOMEDICINE INC.
株式会社ガイアバイオメディシン
Inventors: YONEMITSU Yoshikazu
米満 吉和
HARADA Yui
原田 結
Title: METHOD FOR PREPARING MONONUCLEAR CELLS
Abstract:
The present invention addresses the problem of removing platelets from apheresis blood. Mononuclear cells are prepared while avoiding contamination with platelets. The present invention provides a method for preparing mononuclear cells, said method comprising (1) a step for mixing apheresis blood with a non-cytotoxic and nonionic density controlling agent so as to control the plasma density to 1.066-1.078 g/ml inclusive, and (2) a step for centrifuging a layered product comprising the following layers a and b to thereby separate mononuclear cells: a. a layer of a density gradient centrifugation medium for separating mononuclear cells; and b. a layer of the density-controlled apheresis blood positioned under layer a.