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1. (WO2018045186) METHODS OF COMBINING THE DETECTION OF BIOMOLECULES INTO A SINGLE ASSAY USING FLUORESCENT IN SITU SEQUENCING

Pub. No.:    WO/2018/045186    International Application No.:    PCT/US2017/049641
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Fri Sep 01 01:59:59 CEST 2017
IPC: C12Q 1/68
C12P 19/34
G01N 33/50
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors: CHURCH, George M.
DAUGHARTHY, Evan R.
TERRY, Richard C.
Title: METHODS OF COMBINING THE DETECTION OF BIOMOLECULES INTO A SINGLE ASSAY USING FLUORESCENT IN SITU SEQUENCING
Abstract:
The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.